Department of Pathology
Jim's lab works on development of synthetic circuits and protozoan biology. He leads an EPSRC Global Challenges Research Fund programme to build and employ low cost viral diagnostics, using Synthetic Biology techniques. Jim’s lab also takes on foundational work such as generalised codon optimisation, robust switches and counters and big DNA manipulation, as well as maintain a long interest in protozoan biology. Jim is co-Chair of the University of Cambridge Synthetic Biology Strategic Research Initiative, and co-founder of Colorifix, a startup that is developing sustainable textile dyeing processes, based on synthetic biology.
Synthetic Biology website:
Arsenic Biosensor Collaboration:
Pacini C, Ajioka JW Micklem G (2017) Empirical Bayes method for reducing false discovery rates of correlation matricies with block diagonal structure. BMC Bioinformatics 18(1):213. doi: 10.1186/s12859-017-1623-y.
Juhas M, Ajioka JW (2016) Integrative bacterial artificial chromosomes for DNA integration in the Bacillus subtilis chromosome. J Microbiol Methods. 125:1-7
Juhas M, Wong C, Ajioka JW (2016) Combining genes from multiple phages for improved cell lysis and DNA transfer from Escherichia coli to Bacillus subtilis. PLoS One Oct 31;11(10):e0165778
Juhas M, Ajioka JW (2016) Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome. Microbial Cell Fact 15: 172
Lorenzi H, Khan A, Behnke MS, Namasivayam S, Swanpna LS, Hadjithomas M, Karamycheva S, Pinne D, Brunk B. Ajioka JW, Ajzenberg D, Boothroyd JC, Boyle JP, Darde ML, Diaz-Miranda MA, Dubey JP, Fritz HM, Genean SM, Gregory BD, Kim K, Saeij J, Su C, White MW, Zhu XQ, Howe DK, Rosenthal BM, Grigg ME, Parkinson J, Liu L, Kissinger JC, Roos DS, Sibley, LD (2016) Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes, Nature Comm. 7: 10147
Rudge, TJ, Brown, JR, Federici, F, Dalchau, N, Phillips, A, Ajioka, JW, Haseloff, J (2016) Characterisation of intrinsic properties of promoters. ACS Synthetic Biology 5: 89-98
Juhas M, Evans LD, Frost J, Davenport PW, Yarkoni O, Fraser GM, Ajioka JW (2014) Escherichia coli flagellar genes as target sites for integration and expression of genetic circuits. PLoS One. Oct 28;9(10):e111451
Su C, Khan A, Zhou P, Majumdar D, Ajzenberg D, Dardé ML, Zhu XQ, Ajioka JW, Rosenthal BM, Dubey JP, Sibley LD (2012) Globally diverse Toxoplasma gondii isolates comprise six major clades originating from a small number of distinct ancestral lineages. Proc Natl Acad Sci U S A. 109: 5844-5849.
Dr. Lalitha Sundaram